Intermediate

Part:BBa_K3725060

Designed by: Alice Hou, Michelle Jing, Monica Cho, Janet Standeven, Megan McSweeney   Group: iGEM21_Lambert_GA   (2021-09-29)


Fusarium Trigger Sequence

This part is a fragment of the FRP1 gene of the Fusarium oxysporum species. This fragment is intended to be used in order to interact with the Fusarium Toehold w/ GFP Reporter (Part BBa_K3725020), as well as the Improved Fusarium Toehold w/ GFP Reporter (Part K3725022), to unravel the hairpin loops in the switches, and allow for expression of the GFP reporter proteins downstream. As a result, this part is used to test the functionality of the toehold part in sensing the FRP1 gene and being a working biosensor for the F. oxysporum pathogen.


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None